FFPE RNA/DNA Purification Plus Kit Dx

• Fast and easy processing using rapid spin-column format

• High yields and quality of nucleic acids

• Separate fractionation of RNA and DNA

• Isolate total RNA, from large rRNA down to microRNA (miRNA) 

• No phenol or chloroform extractions

• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples.

FFPE RNA/DNA Purification Plus Kit Dx

Norgen’s FFPE RNA/DNA Purification Plus Kit Dx provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples for subsequent in vitro diagnostic use. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit Dx provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time.

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of nucleic acids followed by signal detection or amplification. Any diagnostic results generated using the RNA and DNA isolated with Norgen’s FFPE RNA/DNA Purification Plus Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s FFPE RNA/DNA Purification Plus Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.

Norgen’s FFPE RNA/DNA Purification Plus Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

Kit Specifications

Maximum Column Binding Capacity (RNA): 35 μg

Maximum Column Binding Capacity (gDNA): 10 μg

Maximum Column Loading Volume: 600 μL

Size of RNA Purified: All sizes, including small RNA (< 200 nt) 

Maximum Amount of Starting Material: 4 sections <20 μM thick 10 mg of unsectioned block

Storage Conditions and Product Stability

The DNAse I and Proteinase K should be stored at -20°C upon arrival. All other solutions and kit components should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels.

Catalog #: Dx54300

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

FFPE RNA/DNA Purification Plus Kit Dx - Protocol (50 preps)

Product Information Sheets

FFPE RNA/DNA Purification Plus Kit - Product Information Sheet

Safety Data Sheets

FFPE RNA/DNA Purification Plus Kit Dx - Safety Data Sheet

Product Flyers

FFPE RNA/DNA Purification Plus Kit