Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples

• Fast and easy processing using rapid spin-column format

• High yields and quality of nucleic acids

• Separate fractionation of RNA and DNA

• Isolate total RNA, from large rRNA down to microRNA (miRNA) 

• No phenol or chloroform extractions

• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

FFPE RNA/DNA Purification Plus Kit

Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The RNA and DNA are sequentially purified into separate fractions using this kit. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.

Kit Specifications

Maximum Binding Capacity: 

- Up to 35 μg RNA
- Up to 10 μg DNA

Maximum Column Loading Volume: 600 μL

Size of RNA Purified: All sizes, including small RNA (< 200 nt) 

Maximum Amount of Starting Material: 4 sections <20 μM thick 10 mg of unsectioned block

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year after the date of shipment.

Catalog #: 54300

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

FFPE RNA/DNA Purification Plus Kit - Protocol (50 prep)
 

Safety Data Sheets

FFPE RNA/DNA Purification Plus Kit - Safety Data Sheet

Product Flyers

FFPE RNA/DNA Purification Plus Kit