For the convenient purification of cytoplasmic and nuclear RNA from cultured cells and tissues

• Excellent separation and purification of cytoplasmic and nuclear RNA

• Convenient and fast spin column format

• High quality and yield of RNA

• Isolate full diversity of RNA (including microRNA) without phenol

• Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more

• Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Cytoplasmic and Nuclear RNA Purification Kit

This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol.   Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations.   Ten samples can be processed in approximately 45 minutes.  This kit is also available in a 100 prep size.    

Background

In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA. 

Kit Specifications

Maximum Column Binding Capacity: Up to 50 µg RNA

Maximum Column Loading Volume: 650 µL

Size of RNA Purified: All sizes, including small RNA (< 200 nt) 

Time to Complete 10 Purifications: 45 minutes

RNA Yield
- HeLa (1 x 10⁶) - Cytoplasmic RNA: 15 µg
- HeLa (1 x 10⁶) - Nuclear RNA: ≤ 3.5 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 21000 -- 50 preps

Catalog #: 37400 -- 100 preps

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Cytoplasmic & Nuclear RNA Purification Kit - Protocol (50 prep)

Cytoplasmic & Nuclear RNA Purification Kit - Protocol (100 prep)

Application Notes

Isolation of Fractionated RNA From Hela Cells Using Norgen's Cytoplasmic Nuclear RNA Purification Kit

 

Safety Data Sheets

Cytoplasmic & Nuclear RNA Purification Kit - Safety Data Sheet

Posters

Cytoplasmic and Nuclear RNA Purification Kit Effects of The Adenovirus Type 5 Tripartite Leader Sequence With Partial and Comple