For convenient purification of RNA and DNA from microorganisms in water samples
Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae
RNA and DNA are both column purified simultaneously using the same column
Elution contains concentrated DNA and RNA without the need for further precipitation
Complete RNA (including microRNA) without phenol
Isolated RNA and DNA are of high quality and integrity for all downstream applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
Water RNA/DNA Purification Kits
These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.
Water RNA/DNA Purification Kit (Spin Column + Filters Kits)
These kits are available with 0.45 μm or 0.22 μm filter formats for capturing microorganisms present in the water.
Kit Specifications
Minimum Water Input: 10 mL
Maximum Water Input: 100 mL
Maximum Filter Column Loading Volume: 20 mL
Maximum Spin Column Loading Volume: 650 µL
Elution Volume: 100 µL
Time to Complete 10 Purifications: 45 minutes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
Catalog #: 26400 -- Spin Column + 0.22 µm Filter
Catalog #: 26450 -- Spin Column + 0.45 µm Filter
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.
Protocols
Water RNA/DNA Purification Kit (Spin Column + 0.22 µm Filter) - Protocol (0.22 µm)
Water RNA/DNA Purification Kit (Spin Column + 0.45 µm Filter) - Protocol (0.45 µm)
Safety Data Sheets
Water RNA/DNA Purification Kit - Safety Data Sheet
Posters
Product Flyers