For the rapid preparation of inhibitor-free total RNA from soil

• Isolate high quality total RNA from a variety of soil samples

• Process all types of soil, including common soil, compost and manure

• Remove all traces of humic acids and other inhibitors of PCR

• Isolates all sizes of RNA, including microRNA, without phenol

• Complete kit including bead tubes and humic acid removal columns (HAR)

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

This kit has a supplementary protocol for the isolation of RNA from Waste Water. See the "Documentation" section for more information.

Soil Total RNA Purification Kit

This kit provides a convenient and rapid spin column method to purify total RNA from small amounts of soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acids and other inhibitors of PCR using humic acid removal (HAR) columns and the organic substance removal solution (OSR).  

Norgen’s Soil Total RNA Purification Kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA without the need for phenol. The purified RNA is of the highest integrity and is inhibitor-free for any downstream application including microbiome studies, real time PCR and reverse transcription PCR for gene expression analysis, Next Generation Sequencing and more.

Kit Specifications

Suggested Soil Input (Clay, loam, sand, feces, compost): 500 mg

Type of Soil Processed: All types, including common soil, compost and manure

Maximum Column Binding Capacity: 50 μg

Maximum Column Loading Volume: 650 μL

Time to Complete Purifications: 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Catalog #: 27750

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Soil Total RNA Purification Kit - Protocol (50 prep)

Supplementary Protocols

Soil RNA Purification Kit - Supplementary Protocol for Isolation of RNA from Waste Water

Application Notes

Preservation of RNA in Soil, Plant and Stool Samples Using Norgen's RNA Preserve

Safety Data Sheets

Soil Total RNA Purification Kit - Safety Data Sheet

Posters

Rapid and qPCR ready DNA isolation from environmental and human bodily fluid samples using Magnetic bead system

Product Flyers

Soil Total RNA Purifi cation Kits