For the rapid purification of microRNA from plants

• Fast and easy processing using a rapid spin-column format

• No phenol or chloroform extractions

• Isolate all small RNA molecules (<200 nt)

• Minimal contamination from large RNA molecules and genomic DNA

• High quality small RNA can be used in various downstream applications

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Plant microRNA Purification Kit

Norgen’s Plant microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured plant cells or plant tissues. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. The small RNA molecules isolated using Norgen’s Plant microRNA Purification Kit can be used in various downstream applications relating to gene regulation and functional analysis, including RT-PCR, miRNA sequencing, northern blotting and microarray analysis.

Kit Specifications

Maximum Column Binding Capacity: 50 μg

Maximum Column Loading Volume: 650 μL

Minimum Elution Volume: 20 μL

Size of RNA Purified: < 200 nt

Amount of Starting Material: 100 mg

- Plant Tissues: 5 x 10⁶ cells

Time to Complete 10 Purifications: 25 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Catalog #: 54700

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Plant microRNA Purification Kit - Protocol (25 prep)

Application Notes

Plant microRNA Purification Kit Evaluation of Plant RNA Integrity Number Rin Generated Using An Agilent Bioanalyzer 2100 - Application Note

Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation

Safety Data Sheets

Plant microRNA Purification Kit - Safety Data Sheet

Posters

The Importance of Sample Preparation For Plant Mirna Purification

Product Flyers

Plant microRNA Purification Kit