For the rapid purification of total RNA (including microRNA) from plants and fungi

• Extract total RNA, including virus & viroid RNA

• Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc

• Isolate total RNA (including microRNA) without phenol

• Isolated RNA is of high quality, integrity and diversity

• Also available in 96-well format for high throughput applications

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Plant/Fungi Total RNA Purification Kit

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient. 

The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.  

Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications.  Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.

Kit Specifications -- Spin Column

* Yield will vary depending on the type of sample processed.
 

Kit Specifications -- High Throughput

* Yield will vary depending on the type of sample processed.
 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 25800 -- Spin Column, 50 preps

Catalog #: 31350 -- Spin Column, 100 preps

Catalog #: 25850 -- Spin Column, 250 preps

Catalog #: 31900 -- High Throughput, 2 x 96-well plates

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Plant/Fungi Total RNA Purification Kit (Spin Column) - Protocol (50 Preps)

Plant/Fungi Total RNA Purification Kit (Spin Column) - Protocol (100 Preps)

Plant/Fungi Total RNA Purification Kit (Spin Column) - Protocol (250 Preps)

Plant/Fungi Total RNA Purification 96-Well Kit (HT) - Protocol (96-well)

Supplementary Protocols

Supplementary Protocol for Plant Tissue Homogenization Without Liquid Nitrogen Grinding

Application Notes

Isolation of Plant and Fungal RNA from Challenging Samples using Norgen’s Plant/Fungi RNA Purification Kit

Non-Organic-Based Isolation of Plant microRNA using Norgen’s Plant/Fungi RNA Purification Kit

Broad Application of a Single Universal Lysis Buffer for True Total RNA Purification from Challenging Plant Species and Tissues

Evaluation of Plant RNA Integrity Number (RIN) generated using an Agilent BioAnalyzer 2100

Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input

Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation

Preservation of RNA in Soil, Plant and Stool Samples Using Norgen's RNA Preserve

Sensitivity and Specificity of Norgen’s HLVd TaqMan RT-PCR Kit.pdf

Safety Data Sheets

Plant/Fungi Total RNA Purification Kit - Safety Data Sheet

Posters

A Novel Technology To Extract Nucleic Acids From Different Environmental Samples Including Plants Water and Soil - Poster

Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - Poster

Compatibility of DNA and RNA Extraction Methods For Challenging Plant Species - Poster

A New Gold Standard for Viroid Detection: Comparison Study Between Membrane Hybridization and RT-PCR

Rapid Isolation and Sensitive Detection of the Chrysanthemum Chlorotic Mottle Viroid

Development of a high throughput PPV detection method based on unique nucleic acid isolation system

The Importance of Sample Preparation for Plant miRNA Purification

Assessing the sensitivity and specificity of three different methods for DNA isolation from Borrelia burgdorferi

Product Flyers

Plant/Fungi Total RNA Purification Kit