For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol
Clean-up & concentrate total RNA (including miRNA) in minutes
Clean-up & concentrate RNA from TRIzol®, TRI Reagent®, etc
Clean-up RNA from contaminants including enzymes, primers, nucleotides
Rapid spin-column protocol, elute in 20 µL
96-well format for high throughput processing & Micro-Elute spin column formats for 8 µL are available
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
RNA Clean-Up and Concentration Kits
These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.
RNA Clean-Up and Concentration Kit (Spin Column)
Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.
RNA Clean-Up and Concentration 96-Well Kit (High Throughput)
Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.
RNA Clean-Up and Concentration Micro-Elute Kit (Micro-Elute)
Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 45 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.
Kit Specifications -- Spin Column
Kit Specifications -- 96-well plate
Kit Specifications -- Micro-Elute
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Catalog #: 23600 -- Spin Column, 50 preps
Catalog #: 43200 -- Spin Column, 100 preps
Catalog #: 25100 -- High Throughput, 2 x 96-well plates
Catalog #: 61000 -- Micro-Elute
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.
Protocols
RNA Clean-Up and Concentration Kit (Spin Column)- Protocol (50 prep)
RNA Clean-Up and Concentration Kit (Spin Column)- Protocol (100 prep)
RNA Clean-Up and Concentration 96-Well Kit (High Throughput) - Protocol (96-well)
RNA Clean-Up and Concentration Micro-Elute Kit (Micro-Elute) - Protocol (50 prep)
Supplementary Protocols
Supplementary Protocol - NGS Library Clean-Up and Concentration
Application Notes
Effect of microRNA GC Content on RNA Purification Efficiency
Safety Data Sheets
RNA Clean-Up and Concentration Kit - Safety Data Sheet
RNA Clean-Up and Concentration 96-Well Kit - Safety Data Sheet
RNA Clean-Up and Concentration Micro-Elute Kit - Safety Data Sheet
Posters
High Expression Level of Hepatitis C Virus E1 and Ns3 Genes - ASM 2009
Effects of Adenovirus Superinfection On Transgene Expression - ASM 2009
Product Flyers