For sequential isolation of total RNA, genomic DNA and total proteins from the same sample

• Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample

• No sample splitting or need to use phenol or precipitation methods

• Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

RNA/DNA/Protein Purification Plus Kits

These kits are ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA/Protein Purification Plus Kits are especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

RNA/DNA/Protein Purification Plus Kit

This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen's resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.

RNA/DNA/Protein Purification Plus Micro Kit

This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.

RNA/DNA/Protein Purification 96-Well Plus Kit

The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen's resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.

Kit Specifications -- Plus, 50 preps (Catalog #: 47700)

Kit Specifications -- Micro Plus, 50 preps (Catalog #: 51600)

Kit Specifications -- High Throughput Plus, 1 x 96-well plate (Catalog #: 51700)

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT).

Catalog #: 47700 -- Plus, 50 preps

Catalog #: 51600 -- Micro Plus, 50 preps

Catalog #: 51700 -- High Throughput Plus, 1 x 96-well plate

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

RNA/DNA/Protein Purification Plus Kit - Protocol (50 prep)

RNA/DNA/Protein Purification Plus Micro Kit - Protocol (50 prep)

RNA/DNA/Protein Purification 96-Well Plus Kit - Protocol (96-well)

Application Notes

Sequential Isolation and Purification of RNA, DNA and Protein from a Single Sample of Animal Cells using Norgen’s RNA/DNA/Protein Purification Kit

Sequential Purification of RNA, DNA and Protein from a Single Sample using Norgen's RNA/DNA/Protein Purification Kit and Comparison to a Market Competitor

Supplementary Protocol for Total RNA Isolation from Staphylococcus aureus-Related Bacteria and Hard-to-Lyse Bacteria Species

Safety Data Sheets

RNA/DNA/Protein Purification Plus Kit - Safety Data Sheet

Product Flyers

RNA/DNA/Protein Purifi cation Plus Kits