For sequential isolation of total RNA and total proteins from the same sample

• Sequentially purify total RNA and total proteins from a single sample

• Kit includes a gDNA elimination column  

• No sample splitting required

• No phenol step required for efficient isolation

• Ideal for small or difficult to obtain samples

• Purify RNA and proteins from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants

• Rapid and efficient spin column procedure

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

RNA/Protein Purification Plus Kit

This kit provides a rapid, single column method for the isolation and purification of total RNA (including miRNA) and proteins sequentially from a single sample of cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants. The total RNA and proteins are both column purified in under 25 minutes using a single column.

Purified RNA is of a high quality and yield, and is suitable for NGS, RT-qPCR and microarrays.  

This kit eliminates DNA efficiently using a gDNA removal column.

Proteins are eluted in buffer and are ready for downstream applications such as Western Blots, Mass Spec and ELISA.  The proteins will not require precipitation, resuspending of pellets, or any further cleaning.

This kit is ideal for researchers who are interested in studying the transcriptome and proteome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Kit Specifications

* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Catalog #: 48200

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

RNA/Protein Purification Plus Kit - Protocol (50 prep)

Safety Data Sheets

RNA/Protein Purification Plus Kit - Safety Data Sheet

Product Flyers

RNA/DNA/Protein Purifi cation Plus Kits