For the rapid extraction and purification of total RNA from leukocytes

• Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer

• Isolate total RNA, including microRNA, without phenol

• Rapid and convenient spin-column format and 96-well plate for high throughput applications

• Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. 

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Leukocyte RNA Purification Kits

These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.

Leukocyte RNA Purification Kit (Spin Column)

This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 10⁶ Leukocytes

Leukocyte RNA Purification Plus Kit (Plus)

Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.

Leukocyte RNA Purification 96-Well Kit (High Throughput)

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen's kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 10⁶ Leukocytes.

Kit Specifications -- Spin Column

Kit Specifications -- Plus

Kit Specifications -- High Throughput

*Additional RBC Lysis Solution is required for input Volumes >150 µL

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The RBC Lysis Buffer should be stored at 4°C upon arrival.  These reagents should remain stable for at least 1 year in their unopened containers.

Catalog #: 21200 -- Spin Column, 50 preps

Catalog #: 21250 -- Plus, 50 preps

Catalog #: 37800 -- High Throughput, 2 x 96-well plates

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Leukocyte RNA Purification Kit (Spin Column) - Protocol (50 prep)

Leukocyte RNA Purification Plus Kit (Plus) - Protocol (50 prep)

Leukocyte RNA Purification 96-Well Kit (High Throughput) - Protocol (96-well)

Application Notes

Rapid Isolation and Purification of Total Leukocyte White Blood Cell RNA From Mammalian Blood Samples

Safety Data Sheets

Leukocyte RNA Purification Kit - Safety Data Sheet

Leukocyte RNA Purification 96-Well Kit - Safety Data Sheet