Rapid purification of total RNA from 5 mL - 10 mL urine without phenol

• Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA

• No phenol extractions

• Very sensitive and linear without the need for carrier RNA

• Bind and elute all RNA irrespective of size or GC content, without bias

• Isolate inhibitor-free urinary RNA

• Slurry/Spin column and Slurry/96-well format available

• resin separation matrix

• Purification is based on Norgen’s proprietary resin matrix

For research use only and NOT intended for in vitro diagnostics.

Urine Total RNA Purification Maxi Kit (Slurry Format)

Norgen’s Urine Total RNA Purification Maxi Kit (Slurry Format) provides a rapid method for the isolation and purification of total RNA from 5 – 10 mL of urine. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies showed that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.

Kit Specifications -- Maxi Slurry

Kit Specifications -- High Throughput Maxi Slurry

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 29600 -- Maxi Slurry, Variable size

Catalog #: 29650 -- High Throughput Maxi Slurry, 1 x 96-well plate

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Urine Total RNA Purification Maxi Kit (Slurry Format) - Protocol (variable)

Urine Total RNA Purification Maxi 96-Well Kit (Slurry Format) - Protocol (96-well)

Application Notes

Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input

Safety Data Sheets

Urine Total RNA Purification Maxi Kit (Slurry Format) - Safety Data Sheet

Urine Total RNA Purification Maxi 96-Well Kit (Slurry Format) - Safety Data Sheet

Product Flyers

Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - ASHG 2011

Differential Expression of Micrornas Assayed From Urine of Patients With Prostate Cancer Or Bph - microRNA 2012

Promising Candidate Urinary Microrna Biomarkers - TriCon 2012