For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine

• Purify all sizes of RNA (including microRNA) without the need for phenol

• Isolate and detect total RNA from 1 mL and up to 50 mL urine

• Provides high-quality RNA for sensitive applications - isolate RNA from as little as 100 cells

• Rapid processing time from sampling to downstream testing

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Urine Exfoliated Cell and Bacteria RNA Purification Kit

This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes.

Background

RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. 

Kit Specifications

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 22550

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Urine Exfoliated Cell and Bacteria RNA Purification Kit - Protocol (25 prep)

Safety Data Sheets

Urine Exfoliated Cell and Bacteria RNA Purification Kit - Safety Data Sheet