Product Overview

The SE Cell Line 4D-Nucleofector™ X Kit S is one of our three 4D-Nucleofector™ Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette™ Strip format when working with the 4D-Nucleofector™ X Unit.

This kit has been determined to be optimal for transfecting following cell types:

• 3T3-L1, diff., 3T3-L1, undiff.

• A-10, A7r5 

• BHK-21, BJ

• C2C12, CaCo-2, Calu-3, Capan-1, Cos-7

• DU145

• GH3

• HCT116, HeLa, HeLa S3

• IMR-90

• Jurkat (DSMZ), Jurkat E6.1

• KG-1a, L6

• MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5

• NCTC clone 929

• Panc-1

• T/G-HA VSMC, T-47D, T84

• U2OS, U-87 MG

• WI-38

The respective cell type-specific Optimized Protocols are available available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

• High transfection efficiencies in difficult-to-transfect cell lines

• Conditions are transferable between 4D Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

• Transfection of low cell numbers in the range of 5 × 10⁴ to 1 × 10⁶ cells

• Medium throughput applications

• Optimization of transfection conditions e.g. substrate titrations

Content

1 x 0.675 mL SE Cell Line Nucleofector^® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0),
2 x 16-well Nucleocuvette^® Strips (20 μL)