Product Overview

The SF Cell Line 4D-Nucleofector™ X Kit L is one of our three 4D-Nucleofector™ Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector™ X Unit.

This kit has been determined to be optimal for transfecting following cell types:

• 293-F (FreeStyle), 32D

• A-20, A2058, A375, A431, A549, AGS, ARPE-19

• BetaTC/6, BxPC-3

• C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1

• EL4

• FDC-P1

• H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C

• IMR-32

• J774A.1

• K-562

• KG-1

• L-428, LNCaP

• MDA-MB-453, MDBK, Molt-4, MV-4-11

• Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0

• P19, P815, PC-12, PC-3

• Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226

• S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480

• T2, T84, TF-1

• U266B1, U-937

• Vero

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

• High transfection efficiencies in difficult-to-transfect cell lines

• Cells maintain functionality post transfection

• Conditions are transferable between 4D-Nucleofector™ System (including the , 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

• Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells

• Low throughput applications 

Content

2.25 mL SF Cell Line Nucleofector™ Solution
0.5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL Nucleocuvette™ Vessel
24 x Single use pipettes