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Yokogawa CellVoyager™ CQ1 Benchtop High-Content Analysis System

CellVoyager™ CQ1 enables 3D imaging and quantification of live cell clusters, such as spheroids within a 3D culture vessel, as they are, keeping the cells intact. CellVoyager™ CQ1 exports feature data in general formats which are readable by various third-party software for advanced data analysis. It is possible to construct a fully customized CellVoyager™ CQ1-based system by integrating with external systems*1, via robot for culture dish handling.

Product description Order information Resources Panel

Overview

 

CellVoyager™ CQ1 System Highlights

 

Excitation laser wavelength   405 nm, 488 nm, 561 nm, 640 nm
Illumination source Laser
Objective lens 2x to 60x
(Dry, Phase contrast, Long working distance)
Camera High-sensitivity sCMOS camera
Autofocus Laser autofocus, Software autofocus
Software CellPathfinder

 

CQ1Enables Measurement of Spheroids, Colonies, and Tissue Sections

 

  • No need to remove cells from the culture dish, in contrast to traditional flow cytometry
  • Nipkow spinning disk confocal technology allows high-speed yet gentle 3D image acquisition
  • Rich feature extraction to facilitate sophisticated cellular image analysis
  • Wide field of view and tiling capability enables easy imaging of large specimen

 

Enables Analysis of Time-Lapse and Live-Cell

 

  • High precision stage incubator and low phototoxicity of our confocal makes the analysis of time-lapse and live-cell are possible   
  • Max.100 fps option for fast time lapse*1

 

 

High-quality Image and similar Operability to a traditional Flow Cytometer

 

  • Feature data and statistical graphs displayed in real-time with image acquisition
  • Usable high-quality image as confocal microscope image.
  • Easy to trace back to the original image from a graph spot, and make repetitive measurements

 

Open Platform

 

  • Connectable with external systems via handling robot*2
  • Expandable to the integrated system as image acquisition and quantification instrument
  • FCS/CSV/ICE data format readable by third-party data analysis software
  • A variety of cell culture and sample dishes are applicable

 

Compact Footprint, lightweight bench-top Device; no Need for a Darkroom

 

  CQ1 General fluorescent imaging Flow cytometry
Cell removal/suspension treatment Not necessary Not necessary Necessary
Cell image confirmation Possible Possible Not possible
Display feature data and graphs in real-time with imaging Possible Depends on devices Possible
3D data measurement Possible Not possible Not possible
Time-lapse Possible Not possible Not possible

*1 Option
*2 Contact to CQ1 partner for more information

 

Details

 

Multiple Functions fully integrated into a compact Box

 

Compact design contains fully integrated multiple functions to offer an easy-to-handle confocal imaging system, without a need for complicated system integration. You only need to set a sample and run the software. A user-friendly interface and versatile functions support your measurement and analysis.

CQ1

 

Principles of the Microlens-enhanced Nipkow Disk Scanning Technology

 

A Nipkow spinning disk containing about 20,000 pinholes and a subsidiary spinning disk containing the same number of microlenses to focus excitation laser light into each corresponding pinhole are mechanically fixed on a motor, and very rapidly rotated. As a result, a high-speed raster scan of the excitation lights on the specimen can be achieved. The pinhole and microlenses are arranged on each disk in our proprietary design to optimize the raster scan. Multi-beam scanning not only increases scanning speed but also results in significantly lower photobleaching and phototoxicity because multi-beam excitation needs only a low level of laser power on the specimen to fully excite fluorescence.

 

CQ1

 

System Integration with CellVoyager™ CQ1

 

System integration with CQ1

 

Analysis software (Option)

 

High-Content Analysis System CellPathfinder™*1        Click Here For More Info!

  • Preset analysis menus for a variety of applications
  • Flexible graph functions to display analysis results
  • A direct link between chart and object image machine learning

    CellPathfinder

 

Machine learning


The software learns the features of the sample objects collected by users.


CellPathfinder

3D Analysis
CellPathfinder

 

Label-free Analysis

 

DPC*2 function is a powerful tool to analyze unstained brightfield samples.

CellPathfinder

*1 Optional software
*2 Digital phase contrast

 

 

Example of Setup

Example of setup

 

Item Specifications
Optics Microlens enhanced dual wide Nipkow disk confocal, transmitted illumination
Laser/Filter Laser: Choose 2-4 lasers from 405/488/561/640 nm,
10-position filter wheel (built-in)
Camera sCMOS 2000 × 2000 pixel, 13.0 × 13.0 mm
Objective lens

Max.6 lenses
(Dry: 2x, 4x, 10x, 20x, 40x; Long working distance: 20x, 40x; Phase contrast: 10x, 20x )

Attachement All wells imaging type, chambered type
Sample vessel Microplate (6, 24, 96, 384 well), slide glass, cover glass chamber, dish (35, 60 mm)
XY stage High-precision XY stage, designated resolution 0.1 µm
Z focus Electric Z motor, designated resolution 0.1 µm
Autofocus Laser autofocus, software autofocus
Feature data Number of cells/cellular granules, intensity, volume, surface area, area, perimeter, diameter, sphericity, circularity, etc
Data format Image: 16bit TIFF file (OME-TIFF), PNG
Numerical data: FCS, CSV, ICE
Workstation Measurement and analysis workstation
Gas Mixer (Option) CO2 concentration: Atmospheric concentration – 7 %
Oconcentration: 3 % – Atmospheric concentration
Size/weight Main unit: 600 × 400 × 437 mm, 44 kg
Utility box: 275 × 432 × 298 mm, 18 kg
Gas Mixer (Option): 170 × 260 × 280 mm, 5.2kg
Environment Main unit and Utility box: 15–35°C, 20–70% RH No condensation Gas Mixer (Option):20–30°C, 10–85% RH No condensation
Power consumption Main unit and Utility box:100-240VAC, 800VA max. Workstation: 100-240 VAC, 400 VA max. Gas Mixer (Option): 100-240 VAC, 40 VA max.

 

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Software