Product Overview

Leukapheresed CD19+ B cells are negatively selected from leukapheresed peripheral blood MNCs using an immunomagnetic labeling system to remove unwanted cell types leaving the B cells untouched. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.

Applications

• Generating antibody libraries

• Co-culture with T helper cells or cytotoxic lymphocytes (CTL)

• B cell activation, clonal expansion, Ig rearrangement, and Ig secretion

• Antigen presenting cell (APC) signal transduction and antigen presentation

Content

1 vial containing ≥ 10 million cryopreserved cells

Recommended Media

Lonza X-VIVO™ 10 with L-glutamine, gentamicin and phenol red

Lonza X-VIVO™ 10 Serum-free Hematopoietic Cell Medium

Lonza X-VIVO™ 15 with L-glutamine, gentamicin, and phenol red

Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium, 500mL

Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium,1L

Lonza X-VIVO™ 15 with with L-glutamine, gentamicin, recombinant transferrin, and phenol red

Catalog #: 4W-601

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Procedure for thawing Poietics™ cells

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved