Lonza CD19+ B Cells [positively selected]
Human CD19+ B cells isolated from peripheral blood using positive immunomagnetic selection, cryopreserved, ≥10 million cells. Catalog #: 4W-600
Product Overview
Leukapheresed CD19+ B Cells are positively selected from leukapheresed peripheral blood MNCs using immunomagnetic CD19 microbeads. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.
Applications
Generating antibody libraries
Co-culture with T helper cells or cytotoxic lymphocytes (CTL)
B cell activation, clonal expansion, Ig rearrangement, and Ig secretion
Antigen presenting cell (APC) signal transduction and antigen presentation
Content
1 vial containing ≥ 10 million cryopreserved cells
Recommended Media
Lonza X-VIVO™ 10 with L-glutamine, gentamicin and phenol red
Lonza X-VIVO™ 10 Serum-free Hematopoietic Cell Medium
Lonza X-VIVO™ 15 with L-glutamine, gentamicin, and phenol red
Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium, 500mL
Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium,1L
Lonza X-VIVO™ 15 with with L-glutamine, gentamicin, recombinant transferrin, and phenol red
Catalog #: 4W-600
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.