Product Overview

Leukapheresed CD14+ monocytes are negatively selected from leukapheresed peripheral blood MNCs using an immunomagnetic labeling system to remove unwanted cell types leaving the monocyte untouched. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols. Manufactured by AllCells^®

Benefits

• Test negative for mycoplasma, bacteria, yeast, and fungi

• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

• Certificate of Analysis (CoA) is provided for each cell lot purchased

Applications

• Generate monocyte-derived M1 and M2 macrophages

• Generate monocyte-derived DCs

• Dendritic cell differentiation and maturation

• Toll-like receptor and pattern recognition receptors (PRR)

• Phagocytosis

• Metabolism mechanisms

Content

1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 10 million cells

Recommended Media

Lonza X-VIVO™ 15 with L-glutamine, gentamicin, and phenol red

Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium, 500mL

Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium,1L

Lonza X-VIVO™ 15 with with L-glutamine, gentamicin, recombinant transferrin, and phenol red

Lonza X-VIVO™ 20 with L-glutamine, gentamicin and phenol red

Lonza X-VIVO™ 20 Serum-free Hematopoietic Cell Medium

Catalog #: 4W-400 -- ≥10 million cells

Catalog #: 4W-400A -- ≥50 million cells

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Procedure for thawing Poietics™ cells

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved