Product Overview

Mobilized peripheral blood CD14+ monocytes are positively selected from mobilized peripheral blood MNC using a direct immunomagnetic CD14 microbeads labeling system. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols. Manufactured by AllCells®

Applications

• Generate monocyte-derived M1 and M2 macrophages

• Generate monocyte-derived DCs

• Dendritic cell differentiation and maturation

• Toll-like receptor and pattern recognition receptors (PRR)

• Phagocytosis

• Metabolism mechanisms

Content

1 vial containing ≥ 10/ 25 million cryopreserved cells

Recommended Media

Lonza X-VIVO™ 15 with L-glutamine, gentamicin, and phenol red

Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium, 500mL

Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium,1L

Lonza X-VIVO™ 15 with with L-glutamine, gentamicin, recombinant transferrin, and phenol red

Lonza X-VIVO™ 20 with L-glutamine, gentamicin and phenol red

Lonza X-VIVO™ 20 Serum-free Hematopoietic Cell Medium

Catalog #: 4Y-125 --  ≥25 million cells

Catalog #: 4Y-125A -- ≥10 million cells

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Procedure for thawing Poietics™ cells

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved