Lonza SG Cell Line 384-well Nucleofector™ Kit
For high-throughput transfection of cell lines, e.g.Ba/F3, Raji or THP-1, when working with the 384-well HT Nucleofector™ System.
Product Overview
The SG Cell Line 384-well Nucleofector™ Kit is one of our three kits suited for high-throughput transfection of cell lines when working with the 384-well HT Nucleofector™ System.
This kit has been determined to be optimal for transfecting following cell types:
Ba/F3
Colo-205
H4
NIH/3T3
Raji, Ramos
THP-1
WEHI
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.
Benefits
High transfection efficiencies in difficult-to-transfect cell lines
Cells maintain functionality post transfection
Seamless integration into automated liquid handling environments
Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System
Applications
Transfection of low cell numbers down to 2 x 104 cell lines
High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Content
2x384 reactions:
22.5 mL SG Cell Line Nucleofector™ Solution
5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 384-well Nucleocuvette™ Plate (20 μl)
10x384 reactions:
90 mL SG Cell Line Nucleofector™ Solution
20 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
10 x 384-well Nucleocuvette™ Plate (20 μl)
Catalog #: V5SC-3002 -- 2x384 reactions
Catalog #: V5SC-3010 -- 10x384 reactions
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.