Product Overview

The Primary Cell Optimization 384-well Nucleofector™ Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector™ System.

Complete Optimization: Different conditions can easily be tested within one experiment using a 384-well Nucleocuvette™ Plate and our pmaxGFP™ Vector as positive control. Our five Primary Cell Nucleofector™ Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. A subsequent optional fine tuning of the Nucleofection conditions can be performed with the help of our scientific support team.

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector™ Solutions and Nucleofector™ Programs. Basic Protocols are available for following cell groups:

• Endothelial cells (mammalian)

• Epithelial cells (mammalian)

• Fibroblasts (mammalian)

• Glial cells (mammalian)

• Neurons (mammalian)

• Smooth muscle cells (mammalian)

• Stem cells (human)

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.

Benefits

• High transfection efficiencies for primary cells

• Automated high-throughput electroporation

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment

• Optimal Nucleofection conditions determined on the 384-well HT Nucleofector™ System are transferable to the 4D-Nucleofector™ System or the 96-well Shuttle™ Add-On

Applications

• Transfection of low cell numbers in the range of 2 x 104 to 1 x 10⁶ cells

• Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector™ System

Content

2.25 mL Nucleofector™ Solution P1

2.25 mL Nucleofector™ Solution P2

2.25 mL Nucleofector™ Solution P3

2.25 mL Nucleofector™ Solution P4

2.25 mL Nucleofector™ Solution P5

4 x 0.15 mL Supplement 1

1 x 0.15 mL Supplement 3

150 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)

1 x 384-well Nucleocuvette™ Plate

Catalog #: V5SP-9001

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Optimization Protocol for Primary Cells - HT Nucleofector™ Device