Product Overview

The Cell Line Optimization 384-well Nucleofector™ Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector™ System.

Different conditions can easily be tested within one experiment using a 384-well Nucleocuvette™ Plate and our pmaxGFP™ Vector as positive control. Our three Cell Line Nucleofector™ Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls. 

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.  

Benefits

• High transfection efficiencies for cell lines

• Automated high-throughput electroporation

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of cell lines within one experiment

• Optimal Nucleofection conditions determined on the 384-well HT Nucleofector™ System are transferable to the 4D-Nucleofector™ System or the 96-well Shuttle™ Add-On

Applications

• Transfection of low cell numbers in the range of 2 x 10⁴ to 1 x 10⁶ cells

• Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 384-well HT Nucleofector™ System

Content

2.25 mL Nucleofector™ Solution SE

2.25 mL Nucleofector™ Solution SF

2.25 mL Nucleofector™ Solution SG

3 x 0.15 mL Supplement 1

150 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)

1 x 384-well Nucleocuvette™ Plate

Catalog #: V5SC-9001

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Cell Line Optimization Protocol - HT Nucleofector™ Device