Genome-Wide Hi-C

There is More to a Genome than Just Sequence

Our Arima-HiC+ Kit provides flexible and robust solutions for exploring both genome sequence + structure so you can understand how spatial relationships in genome structure impact gene regulation, cellular development, and disease processes. 

• Proven Performance: Improve resolution and genome coverage with innovative multiple restriction enzyme chemistry. Save substantial sequencing costs via increased long-range signal.

• User-Friendly Workflow: Gain research insights quickly with rapid 6 hour automation-friendly protocol and reproducible results. Assure library quality with quantitative and predictive QC steps. 

• Sample Flexibility: Analyze previously inaccessible low-input samples, including FFPE, using robust Arima-HiC chemistry. Maximize sample recovery with single-tube chemistry.

Benefits of Genome-Wide HiC
The Arima Genome-Wide HiC+ Kit and workflow is ideal for understanding gene regulation and epigenetic mechanisms.

• - Identify all structural elements in the genome, including compartments, TADs, and loops

• - Achieve uniform coverage across >90% of the genome

• - Utilize a variety of sample types, including plant tissue, cell culture, vertebrate tissue samples, and invertebrates

Product Specifications

Sample Type:

• Tissue, blood, cell lines, whole insects

Sample Input:

• 500K-1M mammalian cells​

• 50-100mg frozen large animal tissue​

• 5-10 mosquitos​

• 2mL of mammalian blood​

• 50-100ul of nucleated blood​

• 125-250mg of plant tissue

Reads:

• Compartments: >50M reads, ​

• TADS: >200M reads,​

• Loops: >600M reads​

Compatible Sequencer:

• Illumina sequencing platforms​

Genome Uniformity:

• ~90% of the genome (8-10% of the genome inaccessible due to repetitive regions)​

Bioinformatics:

• Open-source tools​

 Catalog #: A101070 -- 8 rxns

 Catalog #: A101080 -- 16 rxns

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Product Flyer

The Arima HiC Kit for Reproducible 3D Genome Conformation Analyses

Case Study

Harnessing Multi-Omic Technologies to Reveal Novel Non-Coding Regulatory Elements in Cancer Genomes

Application Brochure

Accelerating Biomarker Discovery, Disease Diagnosis, and Novel Therapies Through Spatial Genomics

User Guide

User Guide: Arima Library Prep for Arima HiC+ Kit

User Guide: Arima HiC Library Preparation using Swift Biosciences Accel-NGS 2S Plus DNA Library Kit

User Guide: Library Preparation using NEBNext Ultra II DNA Library Prep Kit

User Guide: Library Preparation using KAPA Hyper Prep Kit

User Guide: Library Preparation using Illumina TruSeq DNA Nano Library Prep

User Guide: Library Preparation using Active Motif Next Gen DNA Library Kit

User Guide: Arima-HiC for Plant Tissues

User Guide: Arima-HiC for Nucleated Blood

User Guide: Arima-HiC for Mammalian Cell Lines

User Guide: Arima-HiC for Mammalian Blood

User Guide: Arima-HiC for Animal Tissues

QC Worksheet

Worksheet: Arima-HiC Quality Control

Bioinformatics

Bioinformatics User Guide: Arima Structural Variant Pipeline

Bioinformatics User Guide: Arima-HiC and Arima High Coverage HiC

Bioinformatics User Guide: for Visualization of Arima-HiC Data with HiGlass in a Mac or Linux Based OS