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Berthold Sirius 2 Luminometer for Tubes

Intuitive luminescence reading

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The flexible solution

 

Sirius 2 LB 9526 is a compact and highly sensitive tube luminometer. Light collection is maximized through both the close proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. The luminometer is upgradeable with up to two injectors, making it perfect for flash assays, and can be equipped with an intuitive touch screen computer, enabling stand-alone operation. The Sirius 2 supports all basic luminescence technologies including flash-type Luminescence, glow-type Luminescence, bioluminescence and chemiluminescence.

 

Highlights

 

High performance luminescence detection

 

The Sirius 2 detects less than 0.5 zmol of firefly luciferase (1 amol ATP/tube). Light collection is maximized through the close proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. The detector is operated in photon counting mode, which ensures a very low background signal for excellent signal to noise ratio and the highest linearity.

 

Outstanding flexibility

 

The sample chamber is designed for performance and flexibility: from 35 mm petri dishes to microfuge format to 20 mL vials, just pick the sample format that fits your application best. The optional sample holders enable accurate injection into both, microcentrifuge tubes and standard luminescence tubes.

The system is compatible with a variety of sample formats:

  • Standard 12 mm luminescence tubes (55 and 75 mm)
  • Microcentrifuge tubes (1.5 and 2 mL)
  • Scintillation vials (up to 20 mL)
  • 35 mm Petri dishes

 

Intuitive touchscreen operation

 

The touchscreen-driven software is very intuitive and operated by touch gestures. The easy-to-use software provides advanced features, such as handling of replicates, dual assay calculations, and display of graphics.

Data can be downloaded easily onto a USB stick or printed. The results can be exported for further calculation in Excel or other programs.

 

2 screenshots of the Sirius 2 software

 

 

Automatic quickstart

 

Closing of the sample drawer automatically initiates the measurement. There´s no need to press any key. The automatic light shutter protects the detector from ambient light. This makes the system ready for measurement from the moment the door is closed. This means that there is no need to set a long delay before the measurement. This is necessary with most other tube luminometers so that the background can return to the initial level after the door has been opened.

The sample drawer of the Sirius 2

 

Two configurations of the Sirius 2: without injectors and with 2 injectorsFlexible injector solutions

 

The system is available with up to two syringe-type automatic reagent injectors. The injection volume range is 20–500 μL. This very broad volume range allows the Sirius 2 to adapt to any assay using injectors. Ready-to go sample holders for precise injection are available for 1.5 ml and 2 ml microfuge tubes, as well as for 55 mm and 75 mm, 12 mm diameter tubes (standard format for most luminescence assays).

 

 

 

 

 

 

 

Technical Details

 

Measurement

 

Detectors

Low-noise photomultiplier tube in single photon counting mode

Detection technologies

Luminescence

Measurement modes Endpoint, Kinetics, Multiple Kinetics
Spectral range luminescence 300-600 nm

 

Injectors

 

Injectors Up to 2 syringe injectors
Injector volume

20-500 µL

 

Tube formats

 

Tube formats

Luminescence tubes, 12 x 55 mm

Luminescence tubes, 12 x 75 mm

Microcentrifuge tubes, 1.5 and 2 mL

Scintillation vials (up to 20 mL)

35 mm Petri dishes

 

 

Performance

 

Luminescence sensitivity <1 amol ATP/tube (ATP HS assay)
Dynamic range 6 orders of magnitude

 

 

Operation

 

Interface  
  • Windows software, connected to computer by USB
  • Optional integrated computer with built-in software
Display (integrated computer) 7” capacitive touch, gesture control, 480 x 800 pixels
External interfaces (integrated computer) 2x USB 2.0, 1x Ethernet (RJ-45)

Printer interface

(integrated computer)

Network or USB printing (PCL6 or Postscript 3)
Data export CSV (Excel), PDF, HTML formats
Available protocols  
  • PC software: Quick Measurement included; additional options (purchased separately): Single Assay, Dual Assay, Single Kinetics, Multiple Kinetics, Cut-Off
  • Built-in computer: Quick Measurement, Single Assay, Dual Assay

 

Additional Specifications

 

Power Supply 110-240 V, 24 V/2.5 A DC
Temperature tolerance Storage: 0-40° C
Operation: 15-35° C

Humidity tolerance

Storage: up to 80% non-condensing

Operation: up to 70% non-condensing

Dimensions (W x D x H)

349 x 150 x 241 mm

Weight

3.6 kg

 

Download the Product Brochure for more information.

 

For more information or ordering the products, please email us at cs@medikonia.com.