Product Overview

The P3 Primary Cell 4D-Nucleofector™ LV Kit XL* is suited for larger scale transfection of primary cells in the LV Nucleocuvette™ Cartridge format when working with the 4D-Nucleofector™ LV Unit. With the LV Nucleocuvette™ Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.

This kit has been determined to be optimal for transfecting following primary cells and many others that use Solution P3 as optimal Nucleofector™ Solution:

• B cell (human)

• CD34+ cell (human)

• DC (human)

• ES (human), H9

• Macrophage (human)

• Monocyte (human)

• T cell (human), stim.

• T cell (mouse), unstim.

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

*Note: From the five primary cell solutions (P1 – P5) that come with the 4D-Nucleofector™ System only P3 is available for the large-scale formats so far. Solution P3 is suited for most immune and stem cells (e.g. T cells, dendritic cells, CD34 hematopoietic stem cells). For other solutions please contact us.

Benefits

• Closed Nucleofection of up to 1 billion primary cells

• Conditions transferable from existing smaller scale protocols

• Automatic processing via tubing system

• Transfection of up to 20 mL reaction volume in less than 10 minutes

Applications

• For large scale transfection of up to 1 x 10⁹ cells

• Ex-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)

• Generation of large numbers of transiently modified primary cells for cell-based assays

Content

5 x 22.5 mL P3 Primary Cell Nucleofector™ Solution

5 x 5 mL Supplement 1

5 x LV Nucleocuvette™ Cartridge

10 x 4D-Nucleofector™ LV Reservoir

Catalog #: V4LP-3520

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions of use for transfection of unstimulated peripheral blood human T cells with the P3 Primary Cell 4D-Nucleofector™ LV Kit