Product Overview

The SF Cell Line 4D-Nucleofector™ LV Kit XL* is suited for larger scale transfection of cell lines in the LV Nucleocuvette™ Cartridge format when working with the 4D-Nucleofector™ LV Unit. With the LV Nucleocuvette™ Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.

This kit has been determined to be optimal for transfecting following cell lines and many others that use Solution SF as optimal Nucleofector™ Solution:

• HEK293 suspension clones

• CHO-S suspension clones

• K-562

• U-937

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

*Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-Nucleofector™ System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells). For other solutions please contact us.

Benefits

• Closed Nucleofection of up to 10⁹ cells

• Conditions transferable from existing smaller scale protocols

• Automatic processing via tubing system

• Transfection of up to 20 mL reaction volume in less than 10 minutes

Applications

• For large scale transfection of up to 1 x 10⁹ cells

• Transient production of potential therapeutic proteins or antibodies for construct screening

• Generation of large numbers of transiently modified cell lines for cell-based assays

Content

22.5 mL SF Cell Line Nucleofector™ Solution

5 mL Supplement 1

1 x LV Nucleocuvette™ Cartridge

2 x 4D-Nucleofector™ LV Reservoir

Catalog #: V4LC-2020

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions of use for transfection of suspension CHO cells with the 4D-Nucleofector™ LV Unit

Instructions of use for transfection of suspension HEK-293 cells with the 4D-Nucleofector™ LV Unit