Product Overview

The P2 Primary Cell 4D-Nucleofector™ X Kit S is one of our five 4D-Nucleofector™ Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette™ Strip format when working with the 4D-Nucleofector™ X Unit.

This kit has been determined to be optimal for transfecting following cell types:

• Macrophage (mouse)

• NHDF (human), neonatal

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

• High transfection efficiencies for primary cells

• Cells maintain functionality post transfection

• Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

• Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells

• Medium throughput applications

• Optimization of transfection conditions e.g. substrate titrations

Content

1 x 0.675 mL P2 Primary Cell Nucleofector^® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette™ Strips (20 μl)

Catalog #: V4XP-2032

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Transfection Protocol for NHDF-Neo - 4D-Nucleofector™ Device

Basic Transfection Protocol for Fibroblasts - 4D-Nucleofector™ Device