Product Overview

The P2 Primary Cell 96-well Shuttle™ Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 96-well Shuttle™ Add-On.

This kit has been determined to be optimal for transfecting following cell types:

• Macrophage (mouse)

• NHDF (human), neonatal

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.

Benefits

• High transfection efficiencies for primary cells

• Compatible with liquid handling systems for automated high-throughput electroporation

• Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

• Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells

• High-throughput applications,e.g. s creens of arrayed cDNA, RNAi or CRISPR libraries

Content

96 reactions: 

2.25 mL P2 Primary Cell Nucleofector™ Solution
0.5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 96-well Nucleocuvette™ Plate (20 μl)

960 reactions:
22.5 mL P2 Primary Cell Nucleofector™ Solution
5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
10 x 96-well Nucleocuvette™ Plate (20 μl)

Catalog #: V4SP-2096 -- 96 reactions

Catalog #: V4SP-2960 -- 960 reactions

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Transfection Protocol for NHDF-Neo - 96-well Shuttle™ Device

Basic Transfection Protocol for Fibroblasts - 96-well Shuttle™ Device