Lonza P3 Primary Cell 4D-Nucleofector® X Kit L

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Lonza P3 Primary Cell 4D-Nucleofector® X Kit L

For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-Nucleofector™ X Unit (100 µL format).

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Product Overview

The P3 Primary Cell 4D-Nucleofector™ X Kit L is one of our five 4D-Nucleofector™ Kits suited for transfecting primary cells in the 100 µL Nucleocuvette™ Vessel format when working with the 4D-Nucleofector™ X Unit.

This kit has been determined to be optimal for transfecting following cell types: '

B cell (human)
CD34+ cell (human)
Chondrocyte (human)
DC (human)
DC (mouse), mature
ES (human), H9
ES (mouse)
Hepatocyte (human)
HMEC (human)
Keratinocyte (human)
Macrophage (human)
Monocyte (human)
Neuron (rat), brain Hi/Cx
NHBE (human)
RPE (human)
T cell (human), stim.
T cell (human), unstim.
T cell (mouse), unstim.

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.

Benefits

High transfection efficiencies for primary cells
Cells maintain functionality post transfection
Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells
Low throughput applications

Content

12 reactions:

2 x 0.675 mL P3 Primary Cell Nucleofector™ Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
12 x 100 µL Nucleocuvette™Vessel
12 x Single use pipettes

24 reactions:

2.25 mL P3 Primary Cell Nucleofector™ Solution
0.5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL Nucleocuvette™Vessel
24 x Single use pipettes

Catalog #: V4XP-3012 -- 12 reactions

Catalog #: V4XP-3024 -- 24 reactions

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Transfection Protocol for Human Monocyte - 4D-Nucleofector™ Device

Transfection Protocol for Human T Cells (Unstim.) - 4D-Nucleofector™ Device

Transfection Protocol for Mature Mouse DC (BALB/c) - 4D-Nucleofector™ Device

Transfection Protocol for Mouse T Cells (C57BL/6) - 4D-Nucleofector™ Device

Transfection Protocol for Human Macrophages - 4D-Nucleofector™ Device

Transfection Protocol for Human T Cells (Stim.) - 4D-Nucleofector™ Device

Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ Device

Transfection Protocol for Mature Mouse DC (BALB/c) - 4D-Nucleofector™ Device

Basic Transfection Protocol for Neural Cells - 4D-Nucleofector™ Device