Lonza P3 Primary Cell 4D-Nucleofector™ X Kit S

+852 2866 8995

General Enquiry: cs@medikonia.com | Support: support@medikonia.com

Home > Products >Equipment >Transfection

Lonza P3 Primary Cell 4D-Nucleofector™ X Kit S

For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-Nucleofector™ X Unit (20 µL format). Catalog #: V4XP-3032

Product description Order information Resources Panel

Product Overview

The P3 Primary Cell 4D-Nucleofector™ X Kit S is one of our five 4D-Nucleofector™ Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette™ Strip format when working with the 4D-Nucleofector™ X Unit.

This kit has been determined to be optimal for transfecting following cell types:

B cell (human)
CD34+ cell (human)
Chondrocyte (human)
DC (human)
DC (mouse), mature
ES (human), H9
ES (mouse)
Hepatocyte (human)
HMEC (human)
Keratinocyte (human)
Macrophage (human)
Monocyte (human)
Neuron (rat), brain Hi/Cx
NHBE (human)
RPE (human)
T cell (human), stim.
T cell (mouse), unstim.

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

High transfection efficiencies for primary cells
Cells maintain functionality post transfection
Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

Transfection of low cell numbers in the range of 5 x 10⁴to 1 x 10⁶ cells
Medium throughput applications
Optimization of transfection conditions e.g. substrate titrations

Content

1 x 0.675 mL P3 Primary Cell Nucleofector^{^®} Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette^{^®} Strips (20 μl)

Catalog #: V4XP-3032

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Transfection Protocol for Human Monocyte - 4D-Nucleofector™ Device

Transfection Protocol for Human T Cells (Unstim.) - 4D-Nucleofector™ Device

Transfection Protocol for Mature Mouse DC (BALB/c) - 4D-Nucleofector™ Device

Transfection Protocol for Mouse T Cells (C57BL/6) - 4D-Nucleofector™ Device

Transfection Protocol for Human Macrophages - 4D-Nucleofector™ Device

Transfection Protocol for Human T Cells (Stim.) - 4D-Nucleofector™ Device

Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ Device

Transfection Protocol for Mature Mouse DC (BALB/c) - 4D-Nucleofector™ Device

Basic Transfection Protocol for Neural Cells - 4D-Nucleofector™ Device

Transfection Protocol for Human B Cells - 4D-Nucleofector™ Device