Product Overview

The P3 Primary Cell 96-well Shuttle™ Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 96-well Shuttle™ Add-On.

This kit has been determined to be optimal for transfecting following cell types:

• B cell (human)

• CD34+ cell (human)

• Chondrocyte (human)

• DC (human)

• DC (mouse), mature

• ES (human), H9

• ES (mouse)

• Hepatocyte (human)

• HMEC (human)

• Keratinocyte (human)

• Macrophage (human)

• Monocyte (human)

• Neuron (rat), brain Hi/Cx

• NHBE (human)

• RPE (human)

• T cell (human), stim.

• T cell (mouse), unstim.

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.

Benefits

• High transfection efficiencies for primary cells

• Compatible with liquid handling systems for automated high-throughput electroporation

• Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

• Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells 

• High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries

Content

96 reactions: 

• 2.25 mL P3 Primary Cell Nucleofector™ Solution

• 0.5 mL Supplement 1

• 50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)

• 1 x 96-well Nucleocuvette™ Plate (20 μl)

960 reactions:

• 22.5 mL P3 Primary Cell Nucleofector™ Solution

• 5 mL Supplement 1

• 50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)

• 10 x 96-well Nucleocuvette™ Plate (20 μl)

Catalog #: V4SP-3096 -- 96 reactions

Catalog #: V4SP-3960 -- 960 reactions

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Transfection Protocol for Human Monocyte - 4D-Nucleofector™ Device

Transfection Protocol for Human T Cells (Unstim.) - 4D-Nucleofector™ Device

Transfection Protocol for Mature Mouse DC (BALB/c) - 4D-Nucleofector™ Device

Transfection Protocol for Mouse T Cells (C57BL/6) - 4D-Nucleofector™ Device

Transfection Protocol for Human Macrophages - 4D-Nucleofector™ Device

Transfection Protocol for Human T Cells (Stim.) - 4D-Nucleofector™ Device

Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ Device

Transfection Protocol for Mature Mouse DC (BALB/c) - 4D-Nucleofector™ Device

Basic Transfection Protocol for Neural Cells - 4D-Nucleofector™ Device

Transfection Protocol for Human B Cells - 4D-Nucleofector™ Device

Transfection Protocol for Rat Neurons - 4D-Nucleofector™ Device

Transfection Protocol for NHEK-Neo - 4D-Nucleofector™ Device

Transfection Protocol for Mouse ESC - 4D-Nucleofector™ Device

Transfection Protocol for Human ESC - 4D-Nucleofector™ Device

Transfection Protocol for NHEK-Ad - 4D-Nucleofector™ Device

Basic Transfection Protocol for Human Stem Cells - 4D-Nucleofector™ Device

Transfection Protocol for NHBE - 4D-Nucleofector™ Device

Transfection Protocol for Mouse T Cells (BALB/c) - 4D-Nucleofector™ Device

Transfection Protocol for NHAC-kn - 4D-Nucleofector™ Device

Basic Transfection Protocol for Epithelial Cells - 4D-Nucleofector™

Transfection Protocol for HMEC - 4D-Nucleofector™ Device

Transfection Protocol for Human Hepatocytes - 4D-Nucleofector™ Device