Lonza P5 Primary Cell 4D-Nucleofector™ X Kit S
For efficient transfection of specific primary cells, e.g. HUVEC, in the 4D-Nucleofector™ X Unit (20 µL format). Catalog #: V4XP-5032
Product Overview
The P5 Primary Cell 4D-Nucleofector™ X Kit S is one of our five 4D-Nucleofector™ Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette™ Strip format when working with the 4D-Nucleofector™ X Unit.
This kit has been determined to be optimal for transfecting following cell types:
HUVEC
Skeletal Myoblast, hum.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Benefits
High transfection efficiencies for primary cells
Cells maintain functionality post transfection
Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System
Applications
Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
Medium throughput applications
Optimization of transfection conditions e.g. substrate titrations
Content
1 x 0.675 mL P5 Primary Cell Nucleofector® Solution
1 x 0.15 mL Supplement 3
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μl)
Catalog #: V4XP-5032
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.