Lonza SE Cell Line 4D-Nucleofector™ X Kit L
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector™ X Unit (100 µL format).
Product Overview
The SE Cell Line 4D-Nucleofector™ X Kit L is one of our three 4D-Nucleofector™ Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette™ Vessel format when working with the 4D-Nucleofector™ X Unit.
This kit has been determined to be optimal for transfecting following cell types:
3T3-L1, diff., 3T3-L1, undiff.
A-10, A7r5,
BHK-21, BJ
C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
DU145
GH3
HCT116, HeLa, HeLa S3
IMR-90
Jurkat (DSMZ), Jurkat E6.1
KG-1a, L6
MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
NCTC clone 929
Panc-1
T/G-HA VSMC, T-47D
U2OS, U-87 MG
WI-38
T84
The respective cell type-specific Optimized Protocols are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 12 or 24 reactions.
Benefits
High transfection efficiencies in difficult-to-transfect cell lines
Cells maintain functionality post transfection
Conditions are transferable between 4D-Nucleofector™ System (including 4D-Nucleofector™ including LV Unit), 96-well Shuttle™ System and 384-well Nucleofector™ System
Applications
Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
Low throughput applications
Content
12 reactions:
2 x 0.675 mL SE Cell Line Nucleofector™ Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
12 x 100 µL Nucleocuvette™ Vessel,
12 x Single use pipettes
24 reactions:
2.25 mL SE Cell Line Nucleofector™ Solution
0.5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL Nucleocuvette™ Vessel,
24 x Single use pipettes
Catalog #: V4XC-1012 -- 12 reactions
Catalog #: V4XC-1024 -- 24 reactions
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.
DU 145 Transfection Protocol - 4D-Nucleofector™ Device
T84 Transfection Protocol - 4D-Nucleofector™ Device
IMR-90 Transfection Protocol - 4D-Nucleofector™ Device
HeLa S3 Transfection Protocol - 4D-Nucleofector™ Device
MRC-5 Transfection Protocol - 4D-Nucleofector™ Device
MCF7 Transfection Protocol - 4D-Nucleofector™ Device
U-87 MG Transfection Protocol - 4D-Nucleofector™ Device
MG-63 Transfection Protocol - 4D-Nucleofector™ Device
C2C12 Transfection Protocol - 4D-Nucleofector™ Device
Calu-3 Transfection Protocol - 4D-Nucleofector™ Device
3T3-L1 pre-ad Transfection Protocol - 4D-Nucleofector™ Device
Jurkat Transfection Protocol - 4D-Nucleofector™ Device
MDCK Transfection Protocol - 4D-Nucleofector™ Device
HCT 116 Transfection Protocol - 4D-Nucleofector™ Device
HeLa Transfection Protocol - 4D-Nucleofector™ Device
CaCo-2 Transfection Protocol - 4D-Nucleofector™ X-Unit
PANC-1 Transfection Protocol - 4D-Nucleofector™ X-Unit
MEG-01 Transfection Protocol - 4D-Nucleofector™ X-Unit
U-20 S Transfection Protocol - 4D-Nucleofector™ X-Unit
WI-38 Transfection Protocol - 4D-Nucleofector™ X-Unit
3T3-L1 (adipocytes) Transfection Protocol - 4D-Nucleofector™ X-Unit
COS-7 Transfection Protocol - 4D-Nucleofector™ X-Unit
A7r5 Transfection Protocol - 4D-Nucleofector™ X-Unit
GH3 Transfection Protocol - 4D-Nucleofector™ X-Unit
L6 Transfection Protocol - 4D-Nucleofector™ X-Unit