Lonza SE Cell Line 4D-Nucleofector™ X Kit S

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Lonza SE Cell Line 4D-Nucleofector™ X Kit S

For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector™ X Unit (20 µL format). Catalog #: V4XC-1032

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Product Overview

The SE Cell Line 4D-Nucleofector™ X Kit S is one of our three 4D-Nucleofector™ Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette™ Strip format when working with the 4D-Nucleofector™ X Unit.

This kit has been determined to be optimal for transfecting following cell types:

3T3-L1, diff., 3T3-L1, undiff.
A-10, A7r5
BHK-21, BJ
C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
DU145
GH3
HCT116, HeLa, HeLa S3
IMR-90
Jurkat (DSMZ), Jurkat E6.1
KG-1a, L6
MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
NCTC clone 929
Panc-1
T/G-HA VSMC, T-47D, T84
U2OS, U-87 MG
WI-38

The respective cell type-specific Optimized Protocols are available available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

High transfection efficiencies in difficult-to-transfect cell lines
Conditions are transferable between 4D Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

Transfection of low cell numbers in the range of 5 × 10⁴ to 1 × 10⁶ cells
Medium throughput applications
Optimization of transfection conditions e.g. substrate titrations

Content

1 x 0.675 mL SE Cell Line Nucleofector^{^®} Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0),
2 x 16-well Nucleocuvette^{^®} Strips (20 μL)

Catalog #: V4XC-1032

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

DU 145 Transfection Protocol - 4D-Nucleofector™ Device

T84 Transfection Protocol - 4D-Nucleofector™ Device

IMR-90 Transfection Protocol - 4D-Nucleofector™ Device

HeLa S3 Transfection Protocol - 4D-Nucleofector™ Device

MRC-5 Transfection Protocol - 4D-Nucleofector™ Device

MCF7 Transfection Protocol - 4D-Nucleofector™ Device

U-87 MG Transfection Protocol - 4D-Nucleofector™ Device

MG-63 Transfection Protocol - 4D-Nucleofector™ Device

C2C12 Transfection Protocol - 4D-Nucleofector™ Device

Calu-3 Transfection Protocol - 4D-Nucleofector™ Device