Lonza SE Cell Line 96-well Nucleofector™ Kit
For medium-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 96-well Shuttle™ Add-On
Product Overview
The SE Cell Line 96-well Shuttle™ Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 96-well Shuttle™ Add-On.
This kit has been determined to be optimal for transfecting following cell types:
3T3-L1, diff., 3T3-L1, undiff.
A-10, A7r5
BHK-21, BJ
C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
DU145
GH3
HCT116, HeLa, HeLa S3
IMR-90
Jurkat (DSMZ), Jurkat E6.1
KG-1a, L6
MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MG-63, MRC-5
NCTC clone 929
Panc-1
T/G-HA VSMC, T-47D
U2OS, U-87 MG
WI-38
The respective cell type-specific Optimized Protocols or recommendations are available available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.
Benefits
High transfection efficiencies in difficult-to-transfect cell lines
Compatible with liquid handling systems for automated high-throughput electroporation
Conditions are transferable between 4D Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System
Applications
Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Content
96 reactions:
2.25 mL SE Cell Line Nucleofector™ Solution
0.5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 96-well Nucleocuvette™ Plate (20 μL)
960 reactions:
22.5 mL SE Cell Line Nucleofector™ Solution
5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
10 x 96-well Nucleocuvette™ Plate (20 μL)
Catalog #: V4SC-1096 -- 96 reactions
Catalog #: V4SC-1960 -- 960 reactions
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.