Product Overview

The SF Cell Line 4D-Nucleofector™ X Kit S is one of our three 4D-Nucleofector™ Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette™ Strip format when working with the 4D-Nucleofector™ X Unit.

This kit has been determined to be optimal for transfecting following cell types:

• 293-F (FreeStyle), 32D

• A-20, A2058, A375, A431, A549, AGS, ARPE-19

• BetaTC/6, BxPC-3

• C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1

• EL4

• FDC-P1

• H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C

• IMR-32

• J774A.1

• K-562

• KG-1

• L-428, LNCaP

• MDA-MB-453, MDBK, Molt-4, MV-4-11

• Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0

• P19, P815, PC-12, PC-3

• Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226

• S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480

• T2, T84, TF-1

• U266B1, U-937

• Vero

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

• High transfection efficiencies in difficult-to-transfect cell lines

• Cells maintain functionality post transfection

• Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

• Transferability to 4D-Nucleofector™ LV Unit

Applications

• Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells

• Medium throughput applications

• Optimization of transfection conditions e.g. substrate titrations

Content

1 x 0.675 mL SF Cell Line Nucleofector^®; Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette^® Strips (20 μL)

Catalog #: V4XC-2032

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

U-937 Transfection Protocol - 4D-Nucleofector™ Device

CHO-K1 Transfection Protocol - 4D-Nucleofector™ Device

C6 Transfection Protocol - 4D-Nucleofector™ Device

Don't see your cell lines? Please contact us at cs@medikonia.com