Product Overview

The SG Cell Line 4D-Nucleofector™ X Kit L is one of our three 4D-Nucleofector™ Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette™ Vessel format when working with the 4D-Nucleofector™ X Unit.

This kit has been determined to be optimal for transfecting following cell types:

• Ba/F3

• Colo-205, CHO-S

• H4

• NIH/3T3

• Raji, Ramos

• THP-1

• WEHI

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.

Benefits

• High transfection efficiencies in difficult-to-transfect cell lines

• Cells maintain functionality post transfection

• Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

• Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells

• Low throughput applications

Content

12 reactions:

2 x 0.675 mL SG Cell Line Nucleofector™ Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
12 x100 µL Nucleocuvette™ Vessel
12 x Single use pipettes

24 reactions:

2.25 mL SG Cell Line Nucleofector™ Solution
0.5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL Nucleocuvette™ Vessel
24 x Single use pipettes

Catalog #: V4XC-3012 -- 12 reactions

Catalog #: V4XC-3024 -- 24 reactions

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Raji Transfection Protocol - 4D-Nucleofector™ Device

NIH/3T3 Transfection Protocol - 4D-Nucleofector™ Device

Ramos Transfection Protocol - 4D-Nucleofector™ Device

BA/F3 Transfection Protocol - 4D-Nucleofector™ Device

Don't see your cell lines? Please contact us at cs@medikonia.com