Product Overview

The SG Cell Line 4D-Nucleofector^® X Kit S is one of our three 4D-Nucleofector^® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette^® Strip format when working with the 4D-Nucleofector^® X Unit.

This kit has been determined to be optimal for transfecting following cell types:

• Ba/F3

• Colo-205, CHO-S

• H4

• NIH/3T3

• Raji, Ramos

• THP-1

• WEHI

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection^® conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

• High transfection efficiencies in difficult-to-transfect cell lines

• Cells maintain functionality post transfection

• Conditions are transferable between 4D-Nucleofector^® System, 96-well Shuttle^TM System and 384-well Nucleofector^® System

Applications

• Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells

• Medium throughput applications

• Optimization of transfection conditions, e.g. substrate titrations

Content

1 x 0.675 mL SG Cell Line Nucleofector^® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette^® Strips (20 μl)

Catalog #: V4XC-3032

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Raji Transfection Protocol - 4D-Nucleofector™ Device

Ramos Transfection Protocol - 4D-Nucleofector™ Device

THP-1 Transfection Protocol - 4D-Nucleofector™ Device

CHO-S [Suspension] Transfection Protocol - 4D-Nucleofector™ Device

Don't see your cell lines? Please contact us at cs@medikonia.com