Product Overview

The SG Cell Line 96-well Shuttle™ Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 96-well Shuttle™ Add-On.

This kit has been determined to be optimal for transfecting following cell types:

• Ba/F3

• Colo-205

• H4

• NIH/3T3

• Raji, Ramos

• THP-1

• WEHI

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.

Benefits

• High transfection efficiencies in difficult-to-transfect cell lines

• Compatible with liquid handling systems for automated high-throughput electroporation

• Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

• Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells

• High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries

Content

96 reactions:

2.25 mL SG Cell Line Nucleofector™ Solution
0.5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 96-well Nucleocuvette™ Plate (20 μl)

960 reactions:

22.5 mL SG Cell Line Nucleofector™ Solution
5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
10 x 96-well Nucleocuvette™ Plate (20 μl)

Catalog #: V4SC-3096 -- 96 reactions

Catalog #: V4SC-3960 -- 960 reactions

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

NIH/3T3 Transfection Protocol - 96-well Shuttle™ Device

THP-1 Transfection Protocol - 96-well Shuttle™ Device