Product Overview

The Primary Cell Optimization 4D-Nucleofector™ X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector™ X Unit.

With the unique capability of the 4D-Nucleofector™ X Unit to address individual wells of a 16-well Nucleocuvette™ Strip with different programs, primary cell optimizations are easily performed within one experiment.

Complete Optimization: Using five 16-well Nucleocuvette™ Strips and our pmaxGFP™ Vector as positive control, our five Primary Cell Nucleofector™ Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well Nucleocuvette™ Strip can be used for optional fine tuning or performing the first experiment with the selected conditions. Cell Group-Specific Optimization using

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector™ Solutions and Nucleofector™ Programs. Basic Protocols are available for following cell groups:

• Endothelial cells (mammalian)

• Epithelial cells (mammalian)

• Fibroblasts (mammalian)

• Glial cells (mammalian)

• Neurons (mammalian)

• Smooth muscle cells (mammalian)

• Stem cells (human)

Before doing a complete or cell group-specific optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.

Benefits

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment

• Optimal Nucleofection conditions determined in the 16-well Nucleocuvette™ Strips are transferable to the 100 μL single Nucleocuvette™ in the 4D-Nucleofector™ X Unit

• Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

• Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector™ X Unit

Content

• 0.675 mL Nucleofector™ Solution P1

• 0.675 mL Nucleofector™ Solution P2

• 0.675 mL Nucleofector™ Solution P3

• 0.675 mL Nucleofector™ Solution P4

• 0.675 mL Nucleofector™ Solution P5

• 4 x 0.15 mL Supplement 1

• 1 x 0.15 mL Supplement 3

• 50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)

• 6 x 20 µL 16-well Nucleocuvette™ Strips

Catalog #: V4XP-9096

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Optimization Protocol for Primary Cells - 4D-Nucleofector™ Device