Product Overview

Electroporation-based methods have so far required cells to be in suspension for transfection. However, the 4D-Nucleofector™ Y Unit allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in culture can be kept and transfected by Nucleofection in their physiological state.

The Primary Cell Optimization 4D-Nucleofector™ Y Kit is suited for rapid determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector™ Y Unit. Using one 24-well Dipping Electrode Array and our pmaxGFP™ Vector as positive control, our two Primary Cell Nucleofector™ Solutions for adherent Nucleofection (AD1 and AD2) are tested in combination with a pre-selected set of programs plus controls. The remaining 24-well Dipping Electrode Array can be used for optional fine tuning or performing the first experiment with the selected conditions.

The cells are cultured in the 24-well culture plates provided with the kit*. For the transfection step, a 24-well Dipping Electrode Array is inserted after replacing culture medium by the specific Nucleofector™ Solution mixed with the DNA or RNA substrate.

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.

* Other 24-well culture plates may not be compatible with the 24-well Dipping Electrode Array. Please contact our Scientific support if you want to use alternative plates.

Benefits

• Convenient and rapid determination of optimal conditions for Nucleofection of cells in adherence within one experiment

Applications

• Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector™ Y Unit

Content

• 2 x 4.5 mL AD1 4D-Nucleofector™ Y Solution

• 2 x 4.5 mL AD2 4D-Nucleofector™ Y Solution

• 4 x 1 mL Supplement 1

• 6 x 150 μg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)

• 2 x 24-well Dipping Electrode Array

• 2 x Nunclon™ Δ Surface 24-well plate (Nunc)

Catalog #: V4YP-9A48

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Amaxa™ 4D-Nucleofector™ Optimization Protocol for Primary Cells or Cell Lines – Transfection in Adherence