Product Overview

The Primary Cell Optimization 96-well Shuttle™ Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well Shuttle™ Add-On.

With the unique capability of the 96-well Shuttle™ Add-On to address individual wells of a 96-well Nucleocuvette™ Plate with different programs, primary cell optimizations are easily performed within one experiment.

Complete Optimization: Using one 96-well Nucleocuvette™ Plate and our pmaxGFP™ Vector as positive control, our five Primary Cell Nucleofector™ Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well Nucleocuvette™ Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector™ Solutions and Nucleofector™ Programs. Basic Protocols are available for following cell groups:

• Endothelial cells (mammalian)

• Epithelial cells (mammalian)

• Fibroblasts (mammalian)

• Glial cells (mammalian)

• Neurons (mammalian)

• Smooth muscle cells (mammalian)

• Stem cells (human)

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.

Benefits

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment

• Optimal Nucleofection conditions determined on the 96-well Shuttle™ Add-On are transferable to the 4D-Nucleofector™ System or the 384-well HT Nucleofector™ System

Applications

• Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well Shuttle™ Add-On

Content

0.675 mL Nucleofector™ Solution P1

0.675 mL Nucleofector™ Solution P2

0.675 mL Nucleofector™ Solution P3

0.675 mL Nucleofector™ Solution P4

0.675 mL Nucleofector™ Solution P5

4 x 0.15 mL Supplement 1

1 x 0.15 mL Supplement 3

2 x 50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0) 

2 x 96-well Nucleocuvette™ Plates

Catalog #: V4SP-9096

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Optimization Protocol for Primary Cells - 96-well Shuttle™ Device