Product Overview

The Cell Line Optimization 96-well Shuttle™ Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 96-well Shuttle™ Add-On.

With the unique capability of the 96-well Shuttle™ Add-On to address individual wells of a 96-well Nucleocuvette™ Plate with different programs, primary cell optimizations are easily performed within one experiment. Using one 96-well Nucleocuvette™ Plate and our pmaxGFP™ Vector as positive control, our three Cell Line Nucleofector™ Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls.

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.  

Benefits

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment

• Optimal Nucleofection conditions determined on the 96-well Shuttle™ Add-On are transferable to the 4D-Nucleofector™ System or the 384-well HT Nucleofector™ System

Applications

• Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 96-well Shuttle™ Add-On

Content

0.675 mL Nucleofector™ Solution SE

0.675 mL Nucleofector™ Solution SF

0.675 mL Nucleofector™ Solution SG

3 x 0.15 mL Supplement 1

50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)

1 x 96-well Nucleocuvette™ Plate

Catalog #: V4SC-9096

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

siRNA Transfection Protocol - 96-well Shuttle™ Device

Cell Line Optimization Protocol - 96-well Shuttle™ Device