HiChIP

Map the Regulatory Landscape with HiChIP

Combine the power of Arima HiC+ with chromatin immunoprecipitation (ChIP) to discover active gene regulatory interactions so you can better understand gene regulation, study disease mechanisms, and identify novel therapeutic targets.

• Proven Performance: Discover active gene regulatory interactions at high resolution and reveal differential loop calling between sample types.

• Easy-to-Use Workflow: Expedite your time to results with our 2-day workflow and generate reproducible results across cell types and protein targets.

• Increased Efficiency: Detect high-resolution interactions with reduced sequencing depth and combine ChIP and Hi-C experiments into a single workflow

Benefits of Arima-HiC+ for HiChIP

The HiC+ Kit and HiChiP workflow is ideal for a NGS-based assay for multi-omic analyses of chromatin protein binding and associated chromatin interactions to:

• - Target H3K27ac or H3K4me3 using validated antibodies specific for interactions linking active regulatory domains

• - Optimize for ChIP-enriched long-range interactions

• - Uncover cell-type specific chromatin loops associated with active promoters

Product Specifications

Sample Type:

• Mammalian Cells​

• Fresh/Frozen Tissue

Sample Input:

• At least 3-4 M cells​

• Large animal tissue 200-600 mg, fresh frozen 

Validated Antibodies

• H3K27Ac (Active Motif Cat # 91193 or 91194)​

• H3K4me1 (Thermofisher Cat# 710795)​

• H3K4me2 (Active Motif Cat# 39079 or 39679)​

• H3K4me3 (Millipore Cat # 04-745)​

• H3K79me2 (Millipore Cat# 04-835)​

• CTCF (Active Motif Cat # 91285)​

• POLII (Active Motif Cat# 39097 or 39497)​

• Rad21 (Abcam Cat# ab992)

Library Prep:

• IDT Accel-NGS 2S (formerly Swift)​

Compatible Sequencer:

• Illumina sequencing platforms​

Read Depth:

• 1M-2M raw reads, PE 2x150​

• 250-400M reads depending on antibody​

Bioinformatics:

• ​​Arima-MAPS 2.0

Catalog #: A101020

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Sample Prep

Arima-HiC+ Kit -- HiChIP User Guide for Mammalian Cells and Tissues using Transcription Factors and Histones

Library Prep

Arima-HiC+ Kit -- HiChIP Library Preparation using Swift BiosciencesÒ Accel-NGSÒ 2S Plus DNA Library Kit

QC Worksheet

Worksheet: Arima-HiChIP Quality Control

Bioinformatics

Bioinformatics User Guide: Arima HiChIP

Application Note

 Accurate and Cost-effective Discovery of Active Gene Regulatory Interactions

Case Study

HiChIP Reveals Enhancer Hijacking as an Oncogenic Driver of Ambiguous Leukemia